ABO Blood Test to Determine Paternity Essay

The indigen broth test was used in this science laboratory to test which of the terce fathers is the telephone circuit father of Andrea. The blood test that is well related to the blood display case of Andrea is the respective blood father. each(prenominal) blood sample was mixed with semisynthetic anti-A, anti-B, and anti-Rh to test for clotting or not. Father 3 and Andrea were the only subjects tested with Rh positive with all other subjects Rh negative, resulting in Father 3 as the blood father.IntroductionTable 1 Possible Child Blood TypesTable 1 Possible Child Blood TypesThe purpose of this lab is to determine which father is the blood father of the chela. The mother, Andrea, and triple other fathers were tested by their blood pillowcase and Rh factor to determine the blood father. The ABO blood test determines the blood type of subjects by the alleles IA, IB, and i. The blood alleles IA and IB are codominant over i, meaning both of the alleles are dominant over the r ecessive allele i. The antigen with the Rh factor is determined by D as dominant or d as recessive, with the Rh positive factor as dominant and the Rh negative factor as recessive. In the ABO blood test, synthetic blood samples and synthetic serum of Anti-A, Anti-B, and Anti-Rh are mixed respectively into a blood typing slide. If agglutination occurs as the samples are mixed, then the subjects blood type is the respective A, B, or Rh factor. The father with the most closely related alleles with Andrea is the blood father by testing June, the mother, Andrea, and all the fathers.MethodsThe container vials with synthetic blood samples each are respectively the mother, the child, Father 1, Father 2, and Father 3. Each of the respective blood samples are used once ever round in the experiment. One of the blood samples is dropped into the blood typing slide under three labels A, B, and Rh. After applying the blood sample, a drop of synthetic serum blue (anti-A) is dropped into well A. Syn thetic serum yellow (anti-B) is dropped into well B. Synthetic serum go through (anti-Rh) is dropped into well Rh. Using the respective colored toothpicks, each well is stirred and checked for agglutination (clotting). The blood typing slide is washed and re-used for the next blood sample.ResultsTable 2 ABO Test resultsIn Table 2, only Andrea and Father 3 showed agglutination when anti-Rh was mixed with their blood sample. The June and Andrea both showed clotting with anti-B serum. The child showed agglutination in all of the following serums.Table 3 Blood Group Determination TableTable 3 resulted with Andreas blood type assumed as AB negative. Father 3 was the only one with a positive blood type. The Child has a Rh separate of Dd containing the recessive allele d due to her mothers Rh group being homozygous recessive dd. Father 3 had a Rh group of D_ followed with an uknown allele because no known factor can support an exact second allele.DiscussionFather 3 is the blood father of Andrea because in congruity to the lab results. Andrea was tested to have a blood type of AB positive and the mother as B negative. The only possible blood types of a blood father from the results are blood types AB positive or A positive. Only father 3 showed a matching blood type to the following possible blood types. The lab results supported the fact that father 3s blood type was most closely related to Andreas blood type.ReferencesQ, R. (2010, January 5). Paternity testing using ABO blood types is impossible. Retrieved 2010, from Boston Paternity The DNA Solution Blog http//www.bostonpaternity.com/blog/paternity-testing-using-abo-blood-types-is-impossible/Biology 240 lab Handout, Lab 4 Multiple alleles and the ABO blood type